In vitro and In Silico Study of Anti Diabetic Activity on Raphanus sativus Microgreen and Mature Leaf

Abstract

Diabetes is one of the biggest issues the world is currently facing. The leaves of Raphanus sativus methanolic extract were used to screen phytochemicals and antidiabetic effects (by using alpha-amylase inhibition assay). To acquire their crude extracts, the R. sativus leaves were powdered and macerated in methanol after being sun-dried. The percentages of inhibition for the methanolic extracts of R. sativus were determined to be 79.34%, 78.45%, 77.62%, and 74.74%, (respectively), for the microgreen dry, microgreen wet, mature green wet, and mature green dry. Similar results were reported for the inhibitory concentration–50 values of these methanolic extract. When compared to other methanolic extracts, the dry methanolic extract of R. sativus microgreens demonstrated higher potency. In addition, in silico analysis was performed using the using hex 8.0.0 software. Through this identified the binding score of the phytochemical component with alpha amylase enzyme.