Analytical Review: Methods of Determination for Ledipasvir and Velpatasvir in Pharmaceutical and Biological samples

Abstract

Infection due to hepatitis C virus (HCV) is a leading cause for severe chronic liver disease, which can result
in progressive liver damage such as cirrhosis and hepatocellular carcinoma. Thus, it is considered to be a great
worldwide health problem specifically in Egypt, which has the greatest prevalence of the epidemic problem
of HCV in the world in accordance with the reported Egyptian Demographic Health Survey that had reached
14.7%. Hence, prevention of HCV becomes a national priority. Various techniques were used for the analysis of
LDS in pure forms, in their pharmaceutical formulations and in biological fluids. This literature review represents
an up-to-date survey about all reported methods that have been developed for the determination of LDS and VLP
in their pure form, combined form with other drugs, combined form with degradation products, and in biological
samples such as liquid chromatography, spectrophotometry, spectrofluorimetry, and electrochemistry.